Careful consideration should be given to the selection of the species to be used for polyclonal antibody production. S100 antibody staining protocol for immunohistochemistry. Abnormally high levels of s100p are thought to contribute to tumor development and metastatic properties 7. S100 polyclonal primary antibody is a ready to use product that has been specifically optimized for use with bond polymer refine detection. This document contains information for s100, rabbit polyclonal antibody, ivd created date.
S100p was originally described as a placental protein of 95 amino acid residues shares about 50% sequence identity with the brain s100 proteins alpha and beta. In case of an overt reaction, other adjuvants are available for use. Moreover, schwanns cells of the peripheral nervous system are positive. The s100a4 protein belongs to the s100 subfamily, now designated s100a1s100a. S100 proteins are localized in the cytoplasm andor nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. The protein encoded by this gene is a member of the s100 family of proteins containing 2 efhand calciumbinding motifs. S100b belongs to the s100 family, which contain 2 efhand calciumbinding motifs.
Tested in western blot wb, immunofluorescence if, immunocytochemistry icc and. S100 proteins are localized in the cytoplasm andor nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such. Pdf difference between monoclonal and polyclonal antibodies. Cloudclone polyclonal antibody to s100 calcium binding protein a12 s100a12 sku. Readytouse polyclonal rabbit antibody provided in liquid form in a buffer containing stabilizing protein and 0.
Human polyclonal s100 primary antibody for if, ihc abin6711894 2 publications. S100a4 is a calcium binding protein of the s100 family1 and is overexpressed in. S100 calciumbinding protein p, protein s100e, s100e species. S100 from ox brain has been used for the immunization. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0. Use freunds incomplete adjuvant fia and freunds complete adjuvant fca. Mouse anti bovine s100 monoclonal antibody is intended for qualitative immunohistochemistry with normal and neoplastic formalinfixed, paraffinembedded tissue sections, to be viewed by light microscopy. A monoclonal antibody, by contrast, represents antibody from a single antibody producing b cell and therefore only binds with one unique epitope. The s100a1 antibody has been validated for the following applications.
This antibody reacts with bovine, human, mouse, nonhuman primate, rat samples. Tm product data sheet human s100a6 rabbit polyclonal. S100 genes include at least members which are located as a cluster on chromosome 1q21. The protein encoded by this gene is a member of the s100 family of proteins containing 2 efhand calcium. Order cloudclones polyclonal antibody to s100 calcium. Polyclonal antibodies are typically affinity purified, which means that the antibody mixture as opposed to the host animal is exposed to the antigen, and only antibodies that bound the antigen are retained and sent to you. The full protein is reported to be 94 amino acid residues in length.
Clinical interpretation of staining results should be accompanied by histological studies with proper controls. It is also termed as calpactin light chain, p11 or annexin 2 light chain. Check more details about s100a9 polyclonal antibody now. Antis100 antibody produced in rabbit igg fraction of.
Rabbit polyclonal antibody raised against recombinant s100a1. S100 antibody 19 products in validated antibody database. The s100a1 antibody from proteintech is a rabbit polyclonal antibody to a recombinant protein of human s100a1. Z0311 labels glial cells in the brain and ependymal cells. In humans, the s100b protein is encoded by the s100p gene located at 4q16, however most other genes that encode other members of the s100 family are located at 1q21 as a cluster. Affinitypurified rabbit polyclonal antibodies to s100 protein and affinitypurified rabbit polyclonal antibodies to cb1 were manufactured in accordance with the requirements of current good manufacturing practice for drug substances 51 by ab biotechnology edinburgh, united kingdom, licensed by medicines and healthcare products regulatory.
For research use only s100 beta polyclonal antibody. S100 calcium binding protein a10 s100 is located on rat chromosome 2q34. Choose from our s100b polyclonal antibodies and browse our s100b monoclonal antibody catalog. S100p, a member of the s100 family, is a 95 amino acid protein. Antibody to s100 stains schwannomas, ependymomas, astrogliomas, almost all benign and. Tested in immunofluorescence if, immunocytochemistry icc, immunohistochemistry ihc, immunohistochemistry frozen ihc f and immunohistochemistry paraffin ihc p applications. S100 is a reported synonym of the human protein s100 calcium binding protein a1, encoded by the gene s100a1.
S100 protein p concentrated and prediluted polyclonal antibody 901021092017 rev. The demonstration of s100 protein is achieved by first allowing the binding of s100 polyclonal to the section, and then visualizing this binding using the reagents provided in the detection system. These rage polyclonal antibodies were either used individu. This antibody does not cross react with other s100 proteins.
Rozrolimupab is the antirhd recombinant human polyclonal antibody composed of 25 unique igg1 antibodies and is used for the treatment of immune thrombocytopenia purpura and prevention of isoimmunization in rhnegative pregnant women. Accuracy of the determination of s100 protein expression in. Validated in ihc, iccif and tested in mouse, rat, cow, human, monkey. The use of polyclonal antibodies pabs over monoclonal antibodies has its advantages. Its expression is increased in a number of tumors including pancreas, lung, breast, and ovary carcinomas 5 in response to transcriptional activation 6. Nov 04, 2016 while results between the monoclonal and polyclonal antibodies for each epitope were similar, a greater percentage of reads mapped to their expected region of the genome table 4 for the polyclonal antibody to h3k4me3 34% polyclonal mapping to transcription start sites vs. S100b s100 calcium binding protein b is a member of the s100 family of proteins containing 2 efhand calcium binding motifs. The hrpconjugated antirabbit igg antibody gtx211001 was used to detect the primary antibody. For in vitro diagnostic use s100 protein p is a rabbit polyclonal antibody that is intended for laboratory use in the qualitative identification of s100 protein by immunohistochemistry ihc in formalinfixed paraffinembedded. Provide accurate, reproducible results with the thermo scientific s100 a4 ab8, rabbit polyclonal antibody. Polyclonal antibodies an overview sciencedirect topics. Careful consideration should be given to the appropriateness of the species. Antibody to s100 stains schwannomas, ependymomas, astrogliomas, almost all benign.
A specific band was detected for s100a9 at approximately 14 kda as indicated. Each individual antibody in a polyclonal mixture is technically a monoclonal antibody. Jun 26, 2018 affinitypurified rabbit polyclonal antibodies to s100 protein and affinitypurified rabbit polyclonal antibodies to cb1 were manufactured in accordance with the requirements of current good manufacturing practice for drug substances 51 by ab biotechnology edinburgh, united kingdom, licensed by medicines and healthcare products regulatory. Validated in wb, ihc, icc and tested in mouse, rat, human. Thermo scientific lab vision s100 a4 ab8, rabbit polyclonal. The pa516257 immunogen is s100 protein purified from cow brain. S100 protein is also expressed in the antigen presenting cells such as the langerhans cells in skin and interdigitating reticulum cells in the paracortex of lymph nodes. S100b and s100a6 differentially modulate cell survival by. Doseresponse effect of antibodies to s100 protein and.
Immunohistochemical diagnosis relies on a panel of antibodies comprising polyclonal s100 protein and the monoclonal antibodies hmb 45, mart1, tyrosinase and, to a lesser extent, nkic3. S100 proteins are low molecular weight 9 to 14 kda intracellular calciumbinding proteins that control key cellular pathways including regulation of the cytoskeleton, cell migration and adhesion, and host oxidative defense. Antis100 antibody 4b3 astrocyte marker ab14849 abcam. S100 polyclonal antibody catalog number pa516257 product data sheet product specific information pa516257 targets s100 protein in icc and ihc p applications and shows reactivity with bovine, chicken, human, marsupial, mouse, and rat samples.
S100 proteins are localized in the cytoplasm andor nucleus of a wide range of cells, and involved in the regulation of a number of. S100 beta antibody 151461ap thermo fisher scientific. Thermo scientific lab vision s100, rabbit polyclonal antibody. Consult the antibody supplier page for essential details, including target immunogenicity and tested applications. We report here utilization of these monoclonal antibodies to ana lyze s100 localization in several different tumors of the human nervous system.
Background information s100a9 is a calcium binding protein as a member of the s100 family of proteins. Antimouse murine s100 antibody for immunohistochemistry ihc. Request pdf accuracy of the determination of s100 protein expression in malignant melanoma using a polyclonal antibody directed against s100 and monoclonal antibodies specific for s100 alpha and. Product images for s100 polyclonal antibody s100 antibody pa516257 in if immunofluorescence analysis of s100 was performed using 70% confluent log phase a375 cells. Product datasheet s100b polyclonal antibody catalogue code. Systematic comparison of monoclonal versus polyclonal. While results between the monoclonal and polyclonal antibodies for each epitope were similar, a greater percentage of reads mapped to their expected region of the genome table 4 for the polyclonal antibody to h3k4me3 34% polyclonal mapping to transcription start sites vs. In indirect elisa, the antibody shows no reaction with human plasma and cow serum. This protein may function in product datasheet s100b polyclonal antibody catalogue code. The antibody is also a useful aid for classification of malignant melanocytic tumors of the skin and metastases of human malignant melanomas. S100s are a unique group of efhand proteins characterized by celltype and cell cycle specific expression, as well as deregulated expression in neurological disorders s100b alzheimer disease, down syndrome, and epilepsy, inflammatory disorders s100a8a9 cystic fibrosis, arthritis and chronic bronchitis, and certain. Employ one marker in a comprehensive antibody portfolio that addresses all major disease states with the thermo scientific s100, rabbit polyclonal antibody.
Antis100 antibody products antis100 antibodies are readily available from several suppliers. Product datasheet s100b polyclonal antibody catalogue. Antibodies used to identify and locate intracellular and extracellular proteins in common. Antibody to s100 stains schwannomas, ependymomas, astrogliomas, almost all benign and malignant melanomas and their metastases. A read is counted each time someone views a publication summary such as the title, abstract, and list of authors, clicks on a figure, or views or downloads the fulltext. Results aid in the classification of tumors in central and peripheral nervous system, such as schwannomas. Use the list below to choose the s100b antibody which is most appropriate for your research. High affinity and specificity s100a9 polyclonal antibody at. Tested in immunohistochemistry frozen ihc f and immunohistochemistry paraffin ihc p. Thermo scientific lab vision s100, rabbit polyclonal. Tm product data sheet human s100a6 rabbit polyclonal antibody. The antibody was raised in rabbits by immunization with the recombinant human s100 a6. Administer fca only once for the primary injection.
In western blotting of purified human recombinant s100 proteins, the antibody labels s100b strongly, s100a1 weakly, and s100a6 very weakly. No reaction was observed with the other s100 proteins tested, s100a2, s100a3 and s100a4 8. S100 belongs to the family of calcium binding proteins such as calmodulin and troponin c. Counterstained with hematoxylin blue, only nerve and hair follicle are positive. S100 s100 calcium binding protein a10 belongs to the efhandcontaining s100 protein family. This experiment was conducted under reducing conditions and using the 12230 kda separation system. Antis100a4 labels fibroblasts, neuronal structures, tlymphocytes, plasma cells and kupffer cells in normal tissues mol. S100a6 human, rabbit polyclonal antibody biovendor. A large proportion of cells in human tumors originating from different salivary glands are labeled by antis100. S100a9 antibody detects s100a9 protein by western blot analysis. Polyclonal vs monoclonal antibodies pacific immunology. Accuracy of the determination of s100 protein expression.
1293 1554 335 1281 954 1207 959 1156 1068 1205 1558 85 596 621 1198 729 841 326 1402 1185 488 1046 495 1002 186 645 72 1067 359 1411 4 60 1236 1068 981 422 241